perk1 2 (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc manufactures this product 
99
Structured Review
Cell Signaling Technology Inc
perk1 2
Perk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 16846 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+mapk/Phospho-p44%2F42+MAPK+(Erk1%2F2)+(Thr202%2FTyr204)+Antibody/pmc13669479-12-0-8
Average 99 stars, based on 16846 article reviews
Perk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 16846 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+mapk/Phospho-p44%2F42+MAPK+(Erk1%2F2)+(Thr202%2FTyr204)+Antibody/pmc13669479-12-0-8
Average 99 stars, based on 16846 article reviews
perk1 2 - by Bioz Stars,
2026-10
99/100 stars
Images
1) Product Images from "Fc‐Dependent Targeting of the GAS6–MERTK Axis With Monoclonal and Bispecific Antibodies Overcomes Drug Resistance Across EGFR Mutations in Exons 19, 20, and 21"
Article Title: Fc‐Dependent Targeting of the GAS6–MERTK Axis With Monoclonal and Bispecific Antibodies Overcomes Drug Resistance Across EGFR Mutations in Exons 19, 20, and 21
Journal: Advanced Science
doi: 10.1002/advs.78290
Figure Legend Snippet:
Techniques Used: Cell-Signaling
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other:Article Title: ecDNA-driven oncogene super-expressors shape immunoevasive tumor microenvironment Article Snippet: Protein concentration was determined using the BCA Protein Assay Kit (Thermo Fisher Scientific, A53225). Incubation:Article Title: Brain-derived neurotrophic factor scales presynaptic calcium transients to modulate excitatory neurotransmission. Article Snippet: .. Blots were incubated in anti- TrkB 1:100 (BD Transduction, 610101), anti- pTrkB 1:1,000 (EMD Millipore, ABN1381), Article Title: Endosialin promotes vascular maturation by inhibiting Cyr61 expression in melanoma metastasis Article Snippet: Every protein sample was loaded on 8% SDS- PAGE and then transferred into a PVDF membrane (ThermoFisher Scientific). .. And then membrane incubated with primary antibodies overnight at 4°C: anti-EN (#ab48185, Abcam), anti-Cyr61 (#ab230947, Abcam), anti-GAPDH (#10494-1-AP, Proteintech), anti-p-MAPK (#4370T, CST), and Article Title: Vasorin (VASN) overexpression promotes pulmonary metastasis and resistance to adjuvant chemotherapy in patients with locally advanced rectal cancer Article Snippet: The proteins were added to 5x loading buffer (FDbio, Cat# FD006) and boiled at 100 °C for 10 min. Proteins were separated through SDS‒PAGE (EpiZyme) and then transferred to 0.22–0.45 μm PVDF membranes (Merck Millipore). .. The membranes were blocked with 5% nonfat milk for 1 h and incubated with the following primary antibodies: anti-β-actin (Proteintech, Cat# 20536-1-AP, 1:1000), anti-VASN (Abcam, Cat# ab156868, 1:1000), anti-N-cadherin (MCE, Cat# HY-P80238, 1:1000), anti-E-cadherin (MCE, Cat# HY-P80112, 1:1000), anti-β-catenin (MCE, Cat# HY-P80488, 1:1000), anti-CDK1 (Affinity Biosciences, Cat# CDK1/CDC2, 1:1000), anti- Cyclin B1 (Affinity Biosciences, Cat# Cyclin B1, 1:1000), anti-NOTCH1 (CST, Cat# 3608 S, 1:1000), anti-NICD (NOTCH1 intracellular domain, cleaved form of NOTCH1) (CST, Cat# 4147T, 1:1000), Article Title: Vasorin (VASN) overexpression promotes pulmonary metastasis and resistance to adjuvant chemotherapy in patients with locally advanced rectal cancer. Article Snippet: The proteins were added to 5x loading buffer (FDbio, Cat# FD006) and boiled at 100 °C for 10 min. Proteins were separated through SDS‒PAGE (EpiZyme) and then transferred to 0.22–0.45 μm PVDF membranes (Merck Millipore). .. The membranes were blocked with 5% nonfat milk for 1 h and incubated with the following primary antibodies: anti-β-actin (Proteintech, Cat# 20536-1-AP, 1:1000), anti-VASN (Abcam, Cat# ab156868, 1:1000), anti-N-cadherin (MCE, Cat# HY-P80238, 1:1000), anti-E-cadherin (MCE, Cat# HY-P80112, 1:1000), anti-β-catenin (MCE, Cat# HY-P80488, 1:1000), anti-CDK1 (Affinity Biosciences, Cat# CDK1/CDC2, 1:1000), anti- Cyclin B1 (Affinity Biosciences, Cat# Cyclin B1, 1:1000), anti-NOTCH1 (CST, Cat# 3608 S, 1:1000), anti-NICD (NOTCH1 intracellular domain, cleaved form of NOTCH1) (CST, Cat# 4147T, 1:1000), Membrane:Article Title: Endosialin promotes vascular maturation by inhibiting Cyr61 expression in melanoma metastasis Article Snippet: Every protein sample was loaded on 8% SDS- PAGE and then transferred into a PVDF membrane (ThermoFisher Scientific). .. And then membrane incubated with primary antibodies overnight at 4°C: anti-EN (#ab48185, Abcam), anti-Cyr61 (#ab230947, Abcam), anti-GAPDH (#10494-1-AP, Proteintech), anti-p-MAPK (#4370T, CST), and Western Blot:Article Title: Stat3 -mediated Th17 pathogenicity induced by periodontitis contributes to cognitive impairment by promoting microglial M1 polarization Article Snippet: .. Western Blot antibodies: Cell-Signaling:Article Title: Stat3 -mediated Th17 pathogenicity induced by periodontitis contributes to cognitive impairment by promoting microglial M1 polarization Article Snippet: .. Western Blot antibodies: |
![Q209R preferentially engages FAK‐YAP signaling. (A) Western blot analysis of GNAQ, BAP1, Melan‐A, <t>p‐ERK1/2</t> (Thr202/Tyr204), ERK1/2, FRA1, p‐AKT (Ser473), AKT, p‐4EBP1 (Ser65), and 4EBP1 expression in mouse UM cell lines harboring GNAQ Q209R or GNAQ Q209L . HSP90 was used as an internal loading control. (B) Quantification of the relative ratio between p‐AKT (Ser473) and AKT in Q209R and Q209L UM cell lines from (A). (C) Quantification of the relative ratio between p‐4EBP1 (Ser65) and 4EBP1 in Q209R and Q209L UM cell lines from (A). (D) Western blot analysis of p‐FAK (Tyr397), FAK, p‐YAP (Ser127), and YAP in mouse UM cell lines harboring GNAQ Q209R or GNAQ Q209L . HSP90 was used as an internal loading control. (E) Quantification of the relative ratio between p‐FAK (Tyr397) and FAK in Q209R and Q209L UM cell lines from (D). (F) Quantification of the relative ratio between p‐YAP (Ser127) and YAP in Q209R and Q209L UM cell lines from (D). Orthotopic tumor‐derived cell lines (QBM12.1 [Q209R], QBM12.3 [Q209R], QBM24 [Q209R], QBM45 [Q209L], QBM47 [Q209L]) and liver metastatic lines (QBM12L [Q209R], QBM12L2 [Q209R], QBM51L [Q209L], QBM53L [Q209L]) were used in A–F. (G) Representative IHC images showing YAP expression in primary tumors from Gnaq CA ; Bap1 CKO ; Myc CKI mice harboring either GNAQ Q209R or GNAQ Q209L . (H) Quantification of the nuclear‐to‐cytoplasmic YAP intensity ratio (H‐score) among primary tumors from Gnaq CA ; Bap1 CKO ; Myc CKI mice harboring either GNAQ Q209R or GNAQ Q209L . (I) Representative IHC images showing YAP expression in liver metastasis derived from intrasplenic transplantation of mouse UM cell lines harboring GNAQ Q209R or GNAQ Q209L . (J) Quantification of nuclear YAP intensity (H‐score) among liver metastasis derived from intrasplenic transplantation of mouse UM cell lines harboring GNAQ Q209R or GNAQ Q209L .](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5764/pmc13635764/pmc13635764__PCMR-39-0-g001.jpg)
